A cell shrinkage‐induced non‐selective cation conductance with a novel pharmacology in Ehrlich–Lettre‐ascites tumour cells

FEBS Letters

Lawonn, Peter¹  Hougaard, Charlotte²  Wehner, Frank¹  Hoffmann, Else K² 

[1] Max-Planck-Institut für molekulare Physiologie, Otto-Hahn-Strasse 11, D-44227 Dortmund, Germany;August Krogh Institute, Universitetsparken 13, DK-2100 Copenhagen, Denmark

关键词: Ehrlich–Lettre-ascites tumour cell;    Cell volume regulation;    Cation conductance;    Gadolinium;    Benzamil;    Amiloride;   

DOI  :  10.1016/S0014-5793(03)00210-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In whole-cell recordings on Ehrlich–Lettre-ascites tumour (ELA) cells, the shrinkage-induced activation of a cation conductance with a selectivity ratio P _Na:P _Li:P _K:P _choline:P _NMDG of 1.00:0.97:0.88:0.03:0.01 was observed. In order of potency, this conductance was blocked by Gd³⁺=benzamil>amiloride>ethyl-isopropyl-amiloride (EIPA). In patch-clamp studies using the cell-attached configuration, a 14 pS channel became detectable that was reversibly activated upon hypertonic cell shrinkage. It is concluded that ELA cells express a shrinkage-induced cation channel that may reflect a molecular link between amiloride-sensitive and -insensitive channels. In addition, because of its pharmacological profile, it may possibly be related to epithelial Na⁺ channels (ENaCs).

【 授权许可】

Unknown