Selection based on the folding properties of proteins with ribosome display

FEBS Letters

Matsuura, Tomoaki¹  Plückthun, Andreas¹ 

[1] Biochemisches Institut, Universität Zürich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland

关键词: Ribosome display;    In vitro;    Protein folding;    HIC;    hydrophobic interaction chromatography;    PCR;    polymerase chain reaction;    RT;    reverse transcription;   

DOI  :  10.1016/S0014-5793(03)00178-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ribosome display is a powerful tool for selecting and evolving protein functions through ligand-binding. Here, this in vitro system was used to perform selection based on the folding properties of proteins, independent of specific ligand-binding. The selection is based on two properties of misfolded proteins: (1) increased sensitivity to proteolysis and (2) greater exposure of hydrophobic area. By targeting these properties, we show that compactly folded and soluble proteins can be enriched over insoluble and random coil proteins. This approach may be especially useful for selection and evolution of folded proteins from random sequence libraries.

【 授权许可】

Unknown