In vitro selection of other proteins than antibodies by means of ribosome display

FEBS Letters

Erdmann, Volker A¹  Lamla, Thorsten¹ 

[1] Institut für Biochemie, Freie Universität Berlin, Thielallee 63, D-14195 Berlin, Germany

关键词: In vitro translation;    Selection of protein;    Ribosome display;    Affinity tag;   

DOI  :  10.1016/S0014-5793(01)02660-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The in vitro selection and simultaneous evolution of proteins is feasible by means of ribosome display. Here, we describe the use of a protein bearing a binding property without being an antibody for affinity enrichment of the ternary complex, consisting of a protein, a ribosome and a encoding mRNA. The binding property was a simple affinity tag, namely Strep-tag II and His-tag. We could demonstrate that the selection of a specific mRNA encoding a shortened bovine heart fatty acid binding protein with a N-terminal His-tag was possible. After nine cycles of transcription, translation, affinity selection and reverse transcription PCR the protein with the His-tag could be enriched 10⁸-fold.

【 授权许可】

Unknown