Effects of interactions of hepatocyte nuclear factor 4α isoforms with coactivators and corepressors are promoter‐specific

FEBS Letters

Torres-Padilla, Maria Elena¹  Weiss, Mary C¹ 

[1] Unité de Génétique de la Différenciation, FR3 2364 du CNRS, Département de Biologie du Développement, Institut Pasteur, 25 Rue du Dr. Roux, 75724 Paris Cedex 15, France

关键词: Hepatocyte nuclear factor 4α;    Hepatocyte nuclear factor 4α isoform;    Apolipoprotein;    Liver transcription;    Glucocorticoid receptor interacting protein-1;    Silencing mediator for retinoid and thyroid receptors;    HNF4;    HNF4;    hepatocyte nuclear factor;    AF;    activation function;    CBP;    CREB binding protein;    GRIP;    glucocorticoid receptor interacting protein;    SMRT;    silencing mediator for retinoid and thyroid receptors;    Apo;    apolipoprotein;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(03)00174-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding hepatocyte nuclear factor 4α (HNF4α) possesses two alternative promoters responsible for developmental and tissue-specific expression of HNF4α1 and HNF4α7. The two isoforms possess different N-termini and exhibit distinct transactivation properties. We show here for the first time that the effects mediated by HNF4α isoforms in concert with three different coregulators result in promoter-specific responses. Transcript levels of silencing mediator for retinoid and thyroid receptors and glucocorticoid receptor interacting protein-1 in the liver are reduced at birth, a time point when many genes are strongly activated, suggesting that the effects of coregulators on HNF4α activity in vivo could be determined by the levels of their expression as well as by the target promoter.

【 授权许可】

Unknown