In vivo studies of liver‐type fatty acid binding protein (L‐FABP) gene expression in liver of transgenic zebrafish (Danio rerio)

FEBS Letters

Wu, Jen-Leih¹  Her, Guor Mour¹  Chiang, Chia-Chang¹  Chen, Wen-Ya² 

[1] Laboratory of Marine Molecular Biology and Biotechnology, Institute of Zoology, Academia Sinica, Nankang, Taipei 115, Taiwan;Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan

关键词: Hepatogenesis;    Liver primordia;    Morpholino;    Morphant;    Green fluorescent protein;    GFP;    green fluorescent protein;    L-FABP;    liver fatty acid binding protein;   

DOI  :  10.1016/S0014-5793(03)00157-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5′-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5′-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42–51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.

【 授权许可】

Unknown