Identification of genes possibly related to storage root induction in sweetpotato

FEBS Letters

Bae, Jung Myung³  Jeong, Byeong Choon¹  You, Min Kyoung³  Hur, Cheol Goo²  Ahn, Young Sup¹  Suh, Mi Chung³  Shin, Jeong Sheop³ 

[1] Mokpo Experiment Station, National Honam Agricultural Experiment Station, RDA, Muan, Jeollanam-do 534-833, South Korea;National Center for Genome Information, Korea Research Institute of Bioscience and Biotechnology, Daejon 305-333, South Korea;Graduate School of Biotechnology, Korea University, Seoul 136-701, South Korea

关键词: Comparative analysis;    Expressed sequence tag;    Storage root;    Storage root induction;    Sweetpotato;    Ipomoea batatas;    EST;    expressed sequence tag;    MTD1;    methylenetetrahydrofolate dehydrogenase1;   

DOI  :  10.1016/S0014-5793(03)00035-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To identify genes related to initiation of storage root development in sweetpotato, a cDNA library was constructed with early stage storage roots (0.3–1 cm in diameter). Single-pass sequences of the 5′ ends of 2859 sweetpotato cDNA clones were assembled into 483 clusters and 442 singletons. Comparison of sweetpotato expressed sequence tags (ESTs) to nodulation/tumorigenesis-related sequence databases (nodule-, tumor-, potato tuber- and development-related sequences) revealed that homologs of 39 sweetpotato EST sequences potentially involved in gene regulation, signal transduction and development were present in at least one of the nodulation/tumorigenesis-related sequence databases. Northern blot analyses of these 39 sequences identified 22 differentially expressed genes in early stage storage root and fibrous root. These differentially expressed genes will be potential candidates for research to elucidate the molecular processes related to sweetpotato storage root induction.

【 授权许可】

Unknown