Promoter analysis of a medaka fish intestinal guanylyl cyclase gene

FEBS Letters

Suzuki, Norio¹  Nakauchi, Mina¹ 

[1] Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan

关键词: Guanylyl cyclase;    Intestine;    Medaka fish;    cis-Regulatory element;    CACO-2 cell;   

DOI  :  10.1016/S0014-5793(03)00002-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We characterized the promoter activity of a medaka fish intestinal guanylyl cyclase gene, OlGC6, by assay of enzyme activity in response to various promoter–luciferase fusion gene constructs introduced into CACO-2 cells and medaka fish embryos. A transient transfection assay of the various fusion gene constructs showed that the nucleotides between −98 and −89 in the 5′-flanking region of the OlGC6 gene are essential for transcription of the OlGC6 gene in CACO-2, and that the OlGC6 gene fragment between −98 and +50 is sufficient to drive gene expression in the medaka fish intestine. An electrophoretic mobility shift assay and ultraviolet (UV) cross-linking experiments demonstrated that a nuclear protein from CACO-2 cells and the adult medaka fish intestinal cells binds specifically to the AGACCTTTGC nucleotides in the regulatory element.

【 授权许可】

Unknown