In plants the alc gene expression system responds more rapidly following induction with acetaldehyde than with ethanol

FEBS Letters

Chu, Chengcai²  Fernie, Alisdair R.¹  Sonnewald, Uwe²  Junker, Björn H.¹  Willmitzer, Lothar¹ 

[1] Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany;Institut für Pflanzengenetik und Kulturpflanzenforschung, Correnstrasse 3, 06466 Gatersleben, Germany

关键词: Chemical inducer;    Gene expression;    Metabolism;    Ethanol;    Acetaldehyde;   

DOI  :  10.1016/S0014-5793(02)03889-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has recently been proposed that acetaldehyde is the physiological inducer of the alc gene system and hence indirectly the activator of the AlcA promoter in Aspergillus nidulans. Here we show that this chemical induces expression of a GUS (β-D-glucuronidase) reporter under the control of the alc gene system in transgenic potato tubers more rapidly than ethanol allowing tighter control of transgene expression. Furthermore by analysis of metabolite levels we demonstrate that the application of inducer has few effects on metabolism. We propose that this system is therefore ideal for the temporal regulation of important metabolic enzyme activities.

【 授权许可】

Unknown