Candidate markers for stem and early progenitor cells, Musashi‐1 and Hes1, are expressed in crypt base columnar cells of mouse small intestine

FEBS Letters

Fukuzawa, Hiroaki³  Seno, Hiroshi³  Kayahara, Takahisa³  Kageyama, Ryoichiro²  Sawada, Mitsutaka³  Fukui, Hirokazu³  Hiai, Hiroshi⁴  Suzuki, Katsumasa³  Chiba, Tsutomu³  Takaishi, Shigeo³  Okano, Hideyuki¹ 

[1] Department of Physiology, Keio University School of Medicine, Tokyo, Japan;Institute of Virus Research, Kyoto University Graduate School of Medicine, Kyoto, Japan;Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawara-cho, Sakyo-ku, Kyoto 606-8507, Japan;Department of Pathology, Kyoto University Graduate School of Medicine, Kyoto, Japan

关键词: Musashi-1;    Hes1;    Small intestine;    Stem cell;    Crypt base columnar cell;    Msi-1;    Musashi-1;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    (RT-)PCR;    (reverse transcription-)polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(02)03896-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Musashi-1, a neural RNA-binding protein, is important for maintaining neural stem cells. Both Musashi-1 and Hes1, a transcriptional factor regulated by Musashi-1, are expressed in the small intestine. Here we show that Musashi-1 is present in a few epithelial cells just above the Paneth cells in the small intestinal crypt, the putative position of stem cells, whereas Hes1 is expressed in lower crypt cells just above the Paneth cells, including Musashi-1-positive cells. Musashi-1 and Hes1 were not expressed in Paneth cells. Notably, Musashi-1 and Hes1 were coexpressed in the crypt base columnar cells located between the Paneth cells. These findings suggest that not only the cells just above Paneth cells but also the crypt base columnar cells between the Paneth cells have stem cell characteristics.

【 授权许可】

Unknown