A novel zinc finger transcriptional repressor, ZNF224, interacts with the negative regulatory element (AldA‐NRE) and inhibits gene expression

FEBS Letters

Florio, F.²  Izzo, P.²  Monti, M.¹  Costanzo, P.⁴  Pucci, P.¹  Lupo, A.³  Medugno, L.² 

[1] Dipartimento di Chimica Organica e Biochimica, Via Cinthia – Monte S. Angelo, 80126 Naples, Italy;Dipartimento di Biochimica e Biotecnologie Mediche, Facoltà di Medicina e Chirurgia, Università di Napoli ‘Federico II’, Via S. Pansini 5, 80131 Naples, Italy;Dipartimento di Scienze Biologiche ed Ambientali, Facoltà di Scienze, Università del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy;Dipartimento di Medicina Sperimentale e Clinica, Facoltà di Medicina e Chirurgia, Università degli Studi di Catanzaro Magna Graecia, Via T. Campanella 115, 88100 Catanzaro, Italy

关键词: Negative regulatory element;    Matrix-assisted laser desorption ionization;    Zinc finger;    Transcriptional repressor;    Kruppel-associated box domain;    Aldolase A;    AldA-NRE;    aldolase A negative regulatory element;    KRAB;    Kruppel-associated box;    ZNF;    zinc finger;    MALDI;    matrix-assisted laser desorption ionization;    GST;    glutathione S-transferase;   

DOI  :  10.1016/S0014-5793(02)03783-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction between the negative cis-element (AldA-NRE) and p97 repressor nuclear protein is a key step in modulating transcription of the human and mouse aldolase A (AldA) gene during the cell cycle and differentiation. In an attempt to clarify the role of transcriptional repression in regulating gene expression, we purified, from HeLa cells, the nuclear protein that specifically binds to the AldA negative regulatory element (NRE). Matrix-assisted laser desorption ionization-time of flight analysis and examination of protein profiles from the SwissProt database revealed that the previously defined p97 repressor is ZNF224, a zinc finger protein. We demonstrate that ZNF224, a Kruppel-like zinc finger transcription factor, is the repressor protein that specifically binds to the negative cis-element AldA-NRE and affects the AldA-NRE-mediated transcription.

【 授权许可】

Unknown