Lysophosphatidic acid precursor levels decrease and an arachidonic acid-containing phosphatidylcholine level increases in the dorsal root ganglion of mice after peripheral nerve injury

NEUROSCIENCE LETTERS

卷:698

Article

Mihara, Yuki¹  Horikawa, Makoto^2,3  Sato, Shumpei^2,3  Eto, Fumihiro^2,3,7  Hanada, Mitsuru¹  Banno, Tomohiro¹  Arima, Hideyuki¹  Ushirozako, Hiroki¹  Yamada, Tomohiro¹  Xu, Dongmin¹  Okamoto, Ayako¹  Yamazaki, Fumiyoshi^2,3  Takei, Shiro^2,3,4  Omura, Takao¹  Yao, Ikuko⁷  Matsuyama, Yukihiro¹  Setou, Mitsutoshi^2,3,5,6 

[1] Hamamatsu Univ Sch Med, Dept Orthoped Surg, Higashi Ku, 1-20-1 Handayama, Hamamatsu, Shizuoka 4313192, Japan

[2] Hamamatsu Univ Sch Med, Int Mass Imaging Ctr, Higashi Ku, 1-20-1 Handayama, Hamamatsu, Shizuoka 4313192, Japan

[3] Hamamatsu Univ Sch Med, Dept Cellular & Mol Anat, Higashi Ku, 1-20-1 Handayama, Hamamatsu, Shizuoka 4313192, Japan

[4] Chubu Univ, Coll Biosci & Biotechnol, Dept Environm Biol, 1200 Matsumoto Cho, Kasugai, Aichi 4878501, Japan

[5] Preeminent Med Photon Educ & Res Ctr, Higashi Ku, 1-20-1 Handayama, Hamamatsu, Shizuoka 4313192, Japan

[6] Univ Hong Kong, Dept Anat, Pokfulam, 6-F,William MW Mong Block 21 Sassoon Rd, Hong Kong, Peoples R China

[7] Preeminent Med Photon Educ & Res Ctr, Inst Med Photon Res, Dept Opt Imaging, Hamamatsu, Shizuoka, Japan

关键词: Peripheral nerve injury;    Neuropathic pain;    Lysophosphatidic acid;    Sciatic nerve transection;    Matrix-assisted laser desorption ionization;    Imaging mass spectrometry;   

DOI  :  10.1016/j.neulet.2018.12.035

来源: Elsevier

【 摘 要 】

In the current study, we aimed to analyze the lipid changes in the dorsal root ganglion (DRG) after sciatic nerve transection (SNT) using matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). We found that the arachidonic acid-containing phosphatidylcholine (AA-PC), PC(16:0/20:4) largely increased, while PC(16:0/18:1), PC(18:0/18:1) and phosphatidic acid (PA)(36:2) levels largely decreased in the DRG following nerve injury. Previous studies show that the increase in PC(16:0/20:4) was associated with neuropathic pain and that decrease in PC(16:0/18:1), PC(18:0/18:1), and PA(36:2) were due to producing lysophosphatidic acid (LPA), an initiator for neuropathic pain. These results suggest that the lipid changes in DRG after SNT could be the result of changes for the cause of neuropathic pain. Thus, blocking of LPA could be potential for treatment of neuropathic pain.

【 授权许可】

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