Identification of an Arabidopsis mitochondrial succinate–fumarate translocator

FEBS Letters

Schumacher, Karin¹  Frommer, Wolf B.¹  Schwab, Rebecca¹  Hilpert, Melanie¹  Desimone, Marcelo¹  Schwacke, Rainer²  Catoni, Elisabetta¹  Flügge, Ulf-Ingo² 

[1] Plant Physiology, ZMBP, Auf der Morgenstelle 1, 72076 Tübingen, Germany;Botany Institute II, University of Cologne, Gyrhofstraße 15, 50931 Cologne, Germany

关键词: Mitochondrial carrier family;    Succinate transporter;    Anaplerotic reaction;    Gluconeogenesis;    Arabidopsis thaliana;    MCF;    mitochondrial carrier family;   

DOI  :  10.1016/S0014-5793(02)03782-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Complementation of a yeast acr1 mutant carrying a deletion of the succinate/fumarate carrier gene enabled functional identification of a mitochondrial succinate translocator from Arabidopsis thaliana (AtmSFC1). Thus complementation of yeast mutants is applicable also for identification and characterization of organellar transporters. Reverse transcription polymerase chain reaction and promoter-GUS fusion showed expression of AtmSFC1 in 2 day old dark grown seedlings, which declined in cotyledons during further development, consistent with a role in export of fumarate for gluconeogenesis during lipid mobilization at early germination of Arabidopsis seeds. In mature plants, expression was found in developing and germinating pollen, suggesting a role in ethanolic fermentation.

【 授权许可】

Unknown