Endogenous RGS proteins facilitate dopamine D2S receptor coupling to Gαo proteins and Ca2+ responses in CHO‐K1 cells

FEBS Letters

Boutet-Robinet, Elisa A.¹  De Vries, Luc¹  Wurch, Thierry¹  Pauwels, Petrus J.¹  Finana, Frederic¹ 

[1] Department of Cellular and Molecular Biology, Centre de Recherche Pierre Fabre, 17 avenue Jean Moulin, 81106 Castres Cedex, France

关键词: RGS;    Dopamine D2 receptor;    G protein coupling;    GTPγS;    Ca2+ response;    AFU;    arbitrary fluorescence units;    D2S;    D2short;    CHO;    Chinese hamster ovary;    DA;    dopamine;    GAP;    GTPase activating protein;    GPCR;    G protein coupled receptor;    PTX;    pertussis toxin;    D2R;    dopamine D2 receptor;    RGS;    regulator of G protein signalling;    TBS–T;    Tris buffered saline–Tween;    (−)-NPA;    R(−)-propylnorapomorphine;    (+)-NPA;    S(+)-propylnorapomorphine;   

DOI  :  10.1016/S0014-5793(02)03753-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of RGS proteins on dopaminergic D_2S receptor (D_2SR) signalling was investigated in Chinese hamster ovary (CHO)-K1 cells, using recombinant RGS protein- and PTX-insensitive G_αo proteins. Dopamine-mediated [³⁵S]GTPγS binding was attenuated by more than 60% in CHO-K1 D_2SR cells coexpressing a RGS protein- and PTX-insensitive G_αoGly¹⁸⁴Ser:Cys³⁵¹Ile protein versus cells coexpressing a similar amount of PTX-insensitive G_αoCys³⁵¹Ile protein. Dopamine-agonist-mediated Ca²⁺ responses were dependent on the coexpression with a G_αoCys³⁵¹Ile protein and were fully abolished upon coexpression with a G_αoGly¹⁸⁴Ser:Cys³⁵¹Ile protein. These results suggest that interactions between the G_αo protein and RGS proteins are involved in efficient D_2SR signalling.

【 授权许可】

Unknown