Rho‐specific binding and guanine nucleotide exchange catalysis by KIAA0380, a Dbl family member

FEBS Letters

Rümenapp, Ulrich¹  Schwörer, Guntram¹  Jakobs, Karl H.¹  Blomquist, Andrea¹  Psoma, Amalia¹  Schablowski, Helge¹ 

[1] Institut für Pharmakologie, Universitätsklinikum Essen, Hufelandstr. 55, D-45122 Essen, Germany

关键词: RhoA;    Guanine nucleotide exchange factor;    Actin stress fiber;    Lysophosphatidic acid;    J82 bladder carcinoma cell;    DH;    Dbl homology;    GEF;    guanine nucleotide exchange factor;    GST;    glutathione S-transferase;    GTPγS;    guanosine 5′-O-(3-thio)triphosphate;    LPA;    lysophosphatidic acid;    PH;    pleckstrin homology;    RGS;    regulator of G protein signalling;    TRITC;    tetramethylrhodamine isothiocyanate;   

DOI  :  10.1016/S0014-5793(99)01270-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several guanine nucleotide exchange factors (GEFs) for Rho-GTPases have been identified, all of them containing a Dbl homology (DH) and pleckstrin homology (PH) domain, but exhibiting different specificities to the Rho family members, Rho, Rac and Cdc42. We report here that KIAA0380, a protein with a tandem DH/PH domain, an amino-terminal PDZ domain and a regulator of G protein signalling (RGS) homology domain, is a specific GEF for RhoA, but not for Rac1 and Cdc42, as determined by GDP release, guanosine 5′-O-(3-thio)triphosphate (GTPγS) binding and protein binding assays. When expressed in J82 cells, DH/PH domain-containing forms of KIAA0380 induced actin stress fibers, whereas expression of the RGS homology domain prevented lysophosphatidic acid (LPA)-induced stress fiber formation.

【 授权许可】

Unknown