FEBS Letters | |
Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice | |
Tanaka, Akira1  Ohta, Masaru1  Hayakawa, Takahiko1  Nakashima, Asae1  Nakamura, Tatsunosuke2  Hayashi, Yasuyuki1  Hamada, Akira1  | |
[1] Plantech Research Institute, 1000 Kamoshida-cho, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan;Laboratory of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Chiba University, Chiba 263-8522, Japan | |
关键词: Halophyte plants; Sodium/proton antiporter; Rice; Salt tolerance; Transgenic plants; | |
DOI : 10.1016/S0014-5793(02)03679-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by non-stress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na+/H+ antiporter gene in rice plants significantly improves their salt tolerance.
【 授权许可】
Unknown
【 预 览 】
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