Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice

FEBS Letters

Tanaka, Akira¹  Ohta, Masaru¹  Hayakawa, Takahiko¹  Nakashima, Asae¹  Nakamura, Tatsunosuke²  Hayashi, Yasuyuki¹  Hamada, Akira¹ 

[1] Plantech Research Institute, 1000 Kamoshida-cho, Aoba-ku, Yokohama, Kanagawa 227-0033, Japan;Laboratory of Molecular Cell Biology, Faculty of Pharmaceutical Sciences, Chiba University, Chiba 263-8522, Japan

关键词: Halophyte plants;    Sodium/proton antiporter;    Rice;    Salt tolerance;    Transgenic plants;   

DOI  :  10.1016/S0014-5793(02)03679-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na⁺/H⁺ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na⁺/H⁺ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by non-stress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na⁺/H⁺ antiporter gene in rice plants significantly improves their salt tolerance.

【 授权许可】

Unknown