Small interfering RNA and gene silencing in transgenic mice and rats

FEBS Letters

Okabe, Masaru¹  Hasuwa, Hidetoshi¹  Kaseda, Kazuhiro²  Einarsdottir, Thorbjorg¹ 

[1] Genome Information Research Center, Osaka University, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan;Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan

关键词: RNA interference;    Small interfering RNA;    Gene silencing;    RNA polymerase III promoter;    Transgenic animal;   

DOI  :  10.1016/S0014-5793(02)03680-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

After short duplexes of synthetic 21–23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished.

【 授权许可】

Unknown