Effect of annexin I on insulin secretion through surface binding sites in rat pancreatic islets

FEBS Letters

Won, Jong Hak¹  Yoo, Seung-Ah¹  Auh, Chung-Kyoon¹  Hong, Shin-Hee¹  Park, Young Min¹ 

[1] Department of Biological Sciences and Institute for Basic Science, SungKyunKwan University, Suwon 440-746, South Korea

关键词: Annexin I;    Insulin secretion;    Exocytosis;    Annexin I receptor;    Pancreatic islet;    Anx I;    annexin I;    FITC;    fluorescein isothiocyanate;   

DOI  :  10.1016/S0014-5793(02)03613-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study investigates the effect of extracellular annexin I (Anx I) on regulating insulin secretion in isolated rat pancreatic islets. Results show that Anx I stimulates insulin release in pancreatic islets regardless of the presence or absence of extracellular Ca²⁺. In particular, confocal microscopy shows that Anx I binds to the surface of islet cells in the absence of extracellular Ca²⁺. However, insulin secretion through Anx I significantly decreases in trypsin-treated islets. Likewise, there is minimal binding of Anx I to the surface of trypsin-treated islets. Anti-Anx I polyclonal antibody also inhibits the stimulating effect of Anx I on insulin secretion. These results indicate that Anx I is capable of binding to the cell surface receptor, in order to regulate the stimulation of insulin release in rat pancreatic islets.

【 授权许可】

Unknown