ATP depletion increases phosphorylation of elongation factor eEF2 in adult cardiomyocytes independently of inhibition of mTOR signalling

FEBS Letters

Proud, Christopher G¹  McLeod, Laura E¹ 

[1] Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee DD1 5EH, UK

关键词: Protein synthesis;    Elongation;    mTOR;    ATP;    AMP-activated protein kinase;    Cardiomyocyte;    AICAR;    5-aminoimidazole-4-carboxyamide 1-β-D-ribofuranoside;    AMPK;    5′AMP-activated protein kinase;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    eEF2;    eukaryotic elongation factor 2;    eIF;    eukaryotic initiation factor;    mTOR;    mammalian target of rapamycin;    ZMP;    5-aminoimidazole-4-carbonamide riboside monophosphate;   

DOI  :  10.1016/S0014-5793(02)03582-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Translation elongation consumes a high proportion of cellular energy and can be regulated by phosphorylation of elongation factor eEF2 which inhibits its activity. We have studied the effects of ATP depletion on the phosphorylation of eEF2 in adult rat ventricular cardiomyocytes. Energy depletion rapidly leads to inhibition of protein synthesis and increased phosphorylation of eEF2. Stimulation of the AMP-activated protein kinase also causes increases eEF2 phosphorylation. Only at later times is an effect on mTOR signalling observed. These data suggest that energy depletion leads to inhibition of protein synthesis through phosphorylation of eEF2 independently of inhibition of mTOR signalling.

【 授权许可】

Unknown