Differentially expressed genes in sporadic amyotrophic lateral sclerosis spinal cords – screening by molecular indexing and subsequent cDNA microarray analysis

FEBS Letters

Ishigaki, Shinsuke¹  Kato, Kikuya³  Yoshihara, Tsuyoshi¹  Doyu, Manabu¹  Niwa, Jun-ichi¹  Sawada, Ko-ichi¹  Ando, Yoshio¹  Yotsumoto, Yoshihisa²  Yamamoto, Masahiko¹  Sobue, Gen¹ 

[1] Department of Neurology, Nagoya University Graduate School of Medicine, Nagoya 466-0085, Japan;Pharmaceutical Research Division Discovery Technology Laboratory, Mitsubishi Pharma Corporation, Yokohama 227-0033, Japan;Taisho Laboratory of Functional Genomics, Nara Institute of Science and Technology, Nara 630-0101, Japan

关键词: Amyotrophic lateral sclerosis;    Gene expression profile;    Molecular indexing;    Dorfin;    Neugrin;   

DOI  :  10.1016/S0014-5793(02)03546-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To analyze the genes related to the pathophysiology of sporadic amyotrophic lateral sclerosis (SALS) we performed gene profiling of SALS spinal cords using molecular indexing combined with cDNA microarray. Eighty-four fragments were cloned in the first screening procedure with molecular indexing. Subsequent quantitative microarray screening revealed 11 genes which were differentially expressed in SALS. Real-time RT-PCR verified that the expression level of the following six genes was altered in SALS: dorfin, metallothionein-3, 30 kDa TATA-binding protein-associated factor, neugrin, ubiquitin-like protein 5 and macrophage-inhibiting factor-related protein-8. These results indicated that genes associated with the ubiquitin–proteasome system, oxidative toxicity, transcription, neuronal differentiation and inflammation might be involved in the pathogenesis of SALS.

【 授权许可】

Unknown