HspBP1, a homologue of the yeast Fes1 and Sls1 proteins, is an Hsc70 nucleotide exchange factor

FEBS Letters

Raynes, Deborah A¹  Guerriero, Vince²  Brodsky, Jeffrey L³  McLellan, Catherine²  Kabani, Mehdi³ 

[1]Department of Animal Sciences, University of Arizona, Tucson, AZ 85721, USA

[2]Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA

[3]Department of Biological Sciences, 274 Crawford Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA

关键词: HspBP1;    Fes1p;    Sls1p;    Hsc70;    Hsp70;    Nucleotide exchange;   

DOI  :  10.1016/S0014-5793(02)03570-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The yeast FES1 and SLS1 genes encode conserved nucleotide exchange factors that act on the cytoplasmic and endoplasmic reticulum luminal Hsp70s, Ssa1p and BiP, respectively. We report here that mammalian HspBP1 is homologous to Fes1p and that HspBP1 promotes nucleotide dissociation from both Ssa1p and mammalian Hsc70. In contrast, Fes1p inefficiently strips nucleotide from mammalian Hsc70, and unlike HspBP1 does not inhibit chaperone-mediated protein refolding in vitro. Together, our data indicate that HspBP1 is a member of this new class of nucleotide exchange factors that exhibit varying degrees of compartment and species specificity.

【 授权许可】

Unknown