Regulation by heavy metals and temperature of the human BAG‐3 gene, a modulator of Hsp70 activity

FEBS Letters

Pagliuca, Maria Gabriella²  Cigliano, Stefania¹  Lerose, Rosa²  Leone, Arturo² 

[1]Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli ‘Federico II’, Via Sergio Pansini 5, 80131, Napoli, Italy

[2]Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Via Ponte Don Melillo, 84084 Fisciano, Salerno, Italy

关键词: BAG;    Heavy metal;    Cellular stress;    Gene regulation;    Hsp;    heat shock protein;    Hsc70;    constitutive Hsp70 protein;    HSE;    heat shock elements;    MT;    metallothionein;    MRE;    metal regulatory elements;    PLC-γ;    phospholipase C-γ;   

DOI  :  10.1016/S0014-5793(03)00274-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In HeLa cells the expression of the BAG-3 gene, a member of the BAG family, is regulated by heavy metals and temperature, with kinetics of accumulation of mRNAs similar to Hsp70 and metallothioneins. Western blot assays performed with a polyclonal anti-BAG-3 antibody confirmed that higher levels of the protein were present in the cells following heat and metal exposure. By immunofluorescence techniques and cell fractionation assays we demonstrated that following stress BAG-3 protein concentrated in the rough endoplasmic reticulum and associated with the heavy membrane fraction. The role of BAG-3 protein during apoptosis and cellular stress is discussed.

【 授权许可】

Unknown