Expression and function of phospholipase A2 in brain

FEBS Letters

Balboa, Marı́a A¹  Killermann Lucas, Karin¹  Varela-Nieto, Isabel¹  Dennis, Edward A¹ 

[1]Department of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0601, USA

关键词: Brain injury;    Cytokines;    Nervous system;    Human astrocytoma 1231N1;    Inflammation;    Phospholipase A2;    AA;    arachidonic acid;    COX;    cyclooxygenase;    IL-1;    interleukin 1;    LPS;    lipopolysaccharide;    MAFP;    methyl arachidonyl-fluorophosphonate;    PGE2;    prostaglandin E2;    PLA2;    phospholipase A2;    TNF-α;    tumor necrosis factor-α;   

DOI  :  10.1016/S0014-5793(02)03481-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phospholipase A₂ (PLA₂) appears to play a fundamental role in cell injury in the central nervous system. We have investigated PLA₂ expression in the astrocytoma cell line 1231N1, and found that GIVA, GIVB, GIVC and GVI PLA₂ messages are expressed. PLA₂ activity is increased by inflammatory/injury stimuli such as interleukin-1β and lipopolysaccharide in these cells but with very different time courses. The arachidonic acid liberated is converted to prostaglandin E₂, possibly by cyclooxygenase-2, which is induced by inflammatory stimuli. This cell system emerges as a model to study injury/inflammation-related activation of the new PLA₂ forms GIVB and GIVC.

【 授权许可】

Unknown