Testosterone inhibits tumor necrosis factor‐α‐induced vascular cell adhesion molecule‐1 expression in human aortic endothelial cells

FEBS Letters

Hatakeyama, Haruhiko¹  Nakagawa, Atsushi¹  Nakano, Shigeru¹  Nishizawa, Makoto¹  Uchida, Kenzo¹  Kigoshi, Toshikazu¹ 

[1] Department of Internal Medicine, Division of Endocrinology, Kanazawa Medical University, Ishikawa 920-0293, Japan

关键词: Testosterone;    Vascular cell adhesion molecule-1;    Atherosclerosis;    Nuclear factor-κB;    VCAM-1;    vascular cell adhesion molecule-1;    HAEC;    human aortic endothelial cells;    NF-κB;    nuclear factor-κB;    5α-R;    5α-reductase;    T;    testosterone;    5αDHT;    5α-dihydrotestosterone;    AR;    androgen receptor;    TNF-α;    tumor necrosis factor-α;    RT-PCR;    reverse transcription-polymerase chain reaction;    EMSA;    electrophoretic mobility shift assay;   

DOI  :  10.1016/S0014-5793(02)03440-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the effect of testosterone (T) on tumor necrosis factor-α (TNF-α)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) in human aortic endothelial cells. Incubation of these cells with T resulted in a dose-dependent reduction in the expression, with this reduction completely abolished by a selective androgen receptor blocker. Electrophoretic mobility shift assay demonstrated that T inhibited TNF-α-induced activation of the transcriptional nuclear factor-κB, which is critical for the inducible expression of VCAM-1, probably through the suppression of the nuclear translocation. Our results may suggest an inhibitory effect of T on atherogenesis, providing a novel insight into the consideration of the pathogenesis of atherosclerosis.

【 授权许可】

Unknown