The short N‐terminus is required for functional expression of the virus‐encoded miniature K+ channel Kcv

FEBS Letters

DiFrancesco, Dario²  Pagliuca, Cinzia⁴  Moroni, Anna⁴  Gazzarrini, Sabrina⁴  Sangiorgio, Vanessa¹  Viscomi, Carlo¹  Thiel, Gerhard⁶  Van Etten, James L⁵  Meckel, Tobias⁶  Valbuzzi, Paola⁴  Horvath, Ferenc³ 

[1] Department of General Physiology and Biochemistry, Università degli Studi di Milano, Milan, Italy;I.N.F.M. Milano University Unit, Via Celoria 26, 20133 Milan, Italy;Department of Plant Physiology, Faculty of Science, University of Szeged, P.O. Box 654, H-6701 Szeged, Hungary;Department of Biology and Istituto di Biofisica del CNR, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy;Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583-0722, USA;Darmstadt University of Technology, Schnittspahnstrasse 3, 64287 Darmstadt, Germany

关键词: K+ channel;    Chlorella virus PBCV-1;    Kcv;    HEK 293 cell;   

DOI  :  10.1016/S0014-5793(02)03397-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Kcv (K⁺ Chlorella virus) is a miniature virus-encoded K⁺ channel. Its predicted membrane–pore–membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.

【 授权许可】

Unknown