Reg protein is overexpressed in gastric cancer cells, where it activates a signal transduction pathway that converges on ERK1/2 to stimulate growth

FEBS Letters

Kinoshita, Yoshikazu³  Ishihara, Shunji³  Kadowaki, Yasunori³  Kazumori, Hideaki³  Rumi, Mohammed Azharul Karim³  Okamoto, Hiroshi¹  Miyaoka, Youichi³  Sato, Hiroshi³  Chiba, Tsutomu²  Takasawa, Shin¹  Adachi, Kyoichi³ 

[1]Department of Biochemistry, Tohoku University Graduate School of Medicine, Sendai, Japan

[2]Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Kyoto, Japan

[3]Second Department of Internal Medicine, Shimane Medical University, Izumo 693-8501, Japan

关键词: Reg;    Gastric cancer;    Tyrosine kinase;    ERK1/2;    Carcinogenesis;   

DOI  :  10.1016/S0014-5793(02)03398-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Reg is a growth factor with mitogenic effects on pancreatic β cells and gastric stem cells. To date, there has been no information available on Reg-mediated intracellular signal transduction pathways. The role of Reg in the gastric carcinogenesis is also unknown. In the current study, the Reg signaling pathway in gastric cancer cell was examined. Reg treatment of MKN45 gastric cancer cells resulted in tyrosyl-phoshorylation of several cellular proteins and subsequent activation of classical MAPK, ERK1/2. Reg also stimulated thymidine incorporation in MKN45 and AGS gastric cancer cells in a dose-dependent manner. Finally, Reg was shown to be highly expressed in a large number of gastric cancers in vivo. Taken together, these data suggest that gastric cancer cells have gained the ability to overexpress Reg protein, which confer upon themselves added proliferative capacities, resulting in a considerable growth advantage.

【 授权许可】

Unknown