Increased stability of human growth hormone with reduced lactogenic potency

FEBS Letters

Belousova, Yuliya V²  Schulga, Alexey A¹  Lobachov, Vladimir M³  Levichkin, Iliya V¹  Kirpichnikov, Mikhail P¹  Pace, C.Nick⁴  Protasevich, Irina I³  Makarov, Alexander A³ 

[1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St. 17/10, Moscow 117997, Russia;Research Center of Molecular Diagnostics and Therapy, Simpheropolsky Blvd. 8, Moscow 113149, Russia;Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov St. 32, Moscow 119991, Russia;Department of Medical Biochemistry and Genetics, Texas A&M University, College Station, TX 77843, USA

关键词: Growth hormone;    Inclusion bodies;    Lactogenic activity;    Mutagenesis;    Stability;    GH;    growth hormone;    hGH;    human growth hormone;    pGH;    porcine growth hormone;    DSC;    differential scanning calorimetry;    CD;    circular dichroism;    GdnHCl;    guanidine hydrochloride;   

DOI  :  10.1016/S0014-5793(02)03325-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human growth hormone (hGH), whose main function is the somatic growth stimulation, induces diverse effects including lactation. We examined the possibility of hGH stabilization by elimination of its lactogenic activity. Chimeric GHs were constructed by replacement of different segments of hGH with sequences derived from non-lactogenic porcine GH. As was observed in the rat Nb2-11C lymphoma cell test, lactogenic activity of some chimeric hormones was seriously destroyed. This kind of hormones displayed the substantial increase in thermal and guanidine hydrochloride stability. The more stable hGH variants were found to be more soluble in Escherichia coli cells.

【 授权许可】

Unknown