Effect of cholesterol on the interaction of seminal plasma protein, PDC‐109 with phosphatidylcholine membranes

FEBS Letters

Ramakrishnan, M¹  Swamy, Musti J¹  Marsh, D²  Anbazhagan, V¹ 

[1] School of Chemistry, University of Hyderabad, Hyderabad 500 046, India;Max-Planck Institut für biophysikalische Chemie, 37070 Göttingen, Germany

关键词: BSP-A1;    Lipid–protein interaction;    Lipid bilayer;    Cholesterol efflux;    Phosphatidylcholine;    Sphingomyelin;    DMPC;    1;    2-dimyristoyl-sn-glycero-3-phosphocholine;    PC;    phosphatidylcholine;    SM;    sphingomyelin;    EPR;    electron paramagnetic resonance;    14-PCSL;    -PESL;    -PSSL;    1-acyl-2-[14-(4;    4-dimethyloxazolidine-N-oxyl)]stearoyl-sn-glycero-3-phosphocholine;    -phosphoethanolamine;    -phosphoserine;    14-SMSL;    N-[14-(4;    4-dimethyloxazolidine-N-oxyl)stearoyl]-sphingosine-1-phosphocholine;    CSL;    4′;    4′-dimethylspiro(5α-cholestane-3;    2′-oxazolidin)-3′-yloxyl;    ASL;    17β-hydroxy-4′;    4′-dimethylspiro(5α-androstane-3;    2′-oxazolidin)-3′-yloxyl;    Fn-II;    fibronectin type-II domain;    EDTA;    ethylenediaminetetraacetic acid;    Tris;    tris(hydroxymethyl)aminomethane;    HEPES;    N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid);   

DOI  :  10.1016/S0014-5793(02)03316-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Binding of PDC-109, the major protein of the bovine seminal plasma, to sperm plasma membrane results in an efflux of cholesterol and choline phospholipids, a necessary event before capacitation can occur. The selectivity of PDC-109 for different spin-labelled phospholipids and sterol probes in dimyristoylphosphatidylcholine (DMPC) host matrix has been characterized earlier by EPR spectroscopy [Ramakrishnan, M., Anbazhagan, V., Pratap, T.V., Marsh, D. and Swamy, M.J. (2001) Biophys. J. 81, 2215–2225]. In this report the effect of cholesterol on the interaction of PDC-109 with DMPC membranes has been investigated by spin-label EPR spectroscopy. The results indicate that the presence of cholesterol leads to an increased association of different phospholipid as well as sterol probes, thus modulating the interaction of PDC-109 with phospholipid membranes.

【 授权许可】

Unknown