The role of chromophore in the lipid–protein interactions in bacteriorhodopsin–phosphatidylcholine vesicles

FEBS Letters

Yoshihara, Kazuo²  Bryl, Krzysztof¹ 

[1] Department of Physics and Biophysics, University of Warmia and Mazury, 10-957 Olsztyn, Poland;Suntory Institute for Bioorganic Research, SUNBOR, Wakayamadai, Osaka 618, Japan

关键词: Lipid–protein interaction;    Chromophore;    Bacteriorhodopsin;    Lipid phase transition;    Fluorescence excitation spectrum;    BDY;    β-C8-BODIPY 500/510 C5-HPC;    bR;    bacteriorhodopsin;    bO;    bacterioopsin;    PM;    purple membrane;    Ant;    1-acyl-2-[8-(2-anthroyl)-octanoyl]-sn-glycero-3-phosphocholine;    DPH;    1;    6-diphenyl-1;    3;    5-hexatriene;    Lau;    dilauroylglycerophosphocholine;    Myr;    dimyristoylglycerophosphocholine;   

DOI  :  10.1016/S0014-5793(00)01910-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By fluorescence and phase properties of a 1-acyl-2-[8-(2-anthroyl)-octanoyl]-sn-glycero-3-phosphocholine probe, the influence of the chromophore on the phase transition of bacteriorhodopsin–lipid vesicles was investigated. It was observed that removal of the chromophore led to the down-shifting of the phase transition temperatures. The temperatures corresponding to the beginning and ending of the gel–liquid phase transition were also influenced. This demonstrated that the liquid phase is reached more easily when the chromophore is bleached. The results indicate that removal of the chromophore alters the protein–lipid interactions. It is suggested that this alteration might be related to the change in the lipid molecular packing.

【 授权许可】

Unknown