Pulmonary surfactant protein SP‐B is significantly more immunoreactive in anionic than in zwitterionic bilayers

FEBS Letters

Marı́a Oviedo, Jose¹  Casals, Cristina¹  Pérez-Gil, Jesús¹ 

[1] Departamento Bioquı́mica y Biologı́a Molecular I, Facultad Biologı́a, Universidad Complutense, 28040 Madrid, Spain

关键词: Protein epitope;    Enzyme-linked immunosorbent assay;    Membrane protein conformation;    Surface activity;    Lipid–protein interaction;    Ch;    cholesterol;    Chl;    chloroform;    DMAB;    dimethyl aminobenzoic acid;    DPG;    bovine heart diphosphatidylglycerol;    DPPC;    1;    2-dipalmitoyl-sn-glycero-3-phosphocholine;    DPPG;    1;    2-dipalmitoyl-sn-glycero-3-phospho-rac-glycerol;    ECL;    enzyme chemiluminescence;    ELISA;    enzyme-linked immunosorbent assay;    LPC;    1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine;    MBTH;    3-methyl-2-benzothiazolinone hydrazine;    MeOH;    methanol;    PAGE;    polyacrylamide gel electrophoresis;    PA;    egg yolk phosphatidic acid;    Pal;    palmitic acid;    PC;    egg yolk phosphatidylcholine;    PE;    egg yolk phosphatidylethanolamine;    PI;    bovine liver phosphatidylinositol;    PG;    egg yolk phosphatidylglycerol;    PS;    bovine brain phosphatidylserine;    SDS;    sodium dodecyl sulfate;    SP-A;    surfactant protein A;    SP-B;    surfactant protein B;    SP-C;    surfactant protein C;    Ste;    stearic acid;   

DOI  :  10.1016/S0014-5793(01)02350-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Binding of polyclonal and monoclonal antibodies, quantitated by enzyme-linked immunosorbent assay, to porcine SP-B reconstituted in different phospholipid bilayers has been used to assess differences in protein structure due to lipid–protein interactions. SP-B bound significantly more antibodies when it was reconstituted in bilayers made of anionic phospholipids (phosphatidic acid, cardiolipin, phosphatidylglycerol, phosphatidylinositol or phosphatidylserine) than in zwitterionic bilayers (phosphatidylcholine, phosphatidylcholine/cholesterol, or phosphatidylethanolamine) or in fatty acid micelles (made of salts of palmitic or stearic acids). These differences in immunoreactivity can be important in the development of quantitation methods for SP-B in clinical samples based on immunological techniques.

【 授权许可】

Unknown