Proteome analysis of lipofuscin in human retinal pigment epithelial cells

FEBS Letters

Schnölzer, M.³  Schutt, F.¹  Holz, F.G.¹  Kopitz, J.²  Ueberle, B.³ 

[1] Department of Ophthalmology, INF 400, D-69120 Heidelberg, Germany;Department of Pathochemistry and Neurochemistry, University of Heidelberg, INF 220, D-69120 Heidelberg, Germany;Protein Analysis Facility, German Cancer Research Center, INF 280, D-69120 Heidelberg, Germany

关键词: Lipofuscin;    Proteome;    Retinal pigment epithelium;    Age-related macular degeneration;   

DOI  :  10.1016/S0014-5793(02)03312-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Excessive accumulation of lipofuscin in postmitotic retinal pigment epithelial cells is a common pathogenetic pathway in various blinding retinal diseases including age-related macular degeneration, which is now the most common cause of registerable blindness in the industrialized nations. To better understand the role of lipofuscin accumulation and to manipulate the pathogenetic mechanisms on both experimental and therapeutic levels we analyzed the proteome of isolated human ocular lipofuscin granules from human RPE cells. After homogenization and fractionation by gradient ultracentrifugation of the RPE/choroid complex from 10 pairs of human donors, protein compounds were separated by 2D gel electrophoresis and analyzed using matrix-assisted laser desorption/ionization mass spectrometry and HPLC-coupled electrospray tandem mass spectrometry. Besides a better understanding of downstream pathways, this approach may provide new targets for therapeutic interventions in a currently untreatable disease.

【 授权许可】

Unknown