Characterization of a general amino acid permease from Hebeloma cylindrosporum

FEBS Letters

Frommer, Wolf B¹  Benjdia, Mariam¹  Wipf, Daniel¹  Tegeder, Mechthild¹ 

[1] ZMBP, Plant Physiology, Auf der Morgenstelle 1, D-72076 Tübingen, Germany

关键词: Amino acid transport;    Aspartate uptake;    Hebeloma cylindrosporum;   

DOI  :  10.1016/S0014-5793(02)03271-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Besides a role in phosphate supply, ectomycorrhizas play a crucial role in nitrogen nutrition of plants. The ectomycorrhizal association between Hebeloma cylindrosporum and Pinus pinaster serves as a model system accessible to molecular manipulation. Hebeloma mycelium is able to take up and use amino acids as the sole nitrogen source. Suppression cloning allowed identification of a Hebeloma transporter (HcGAP1) mediating histidine uptake. HcGAP1 mediates secondary active uptake of a wide spectrum of different amino acids. The secondary active transport mechanism together with the expression in hyphae, but not in mycorrhizas, indicate a role in uptake of organic nitrogen from the soil.

【 授权许可】

Unknown