期刊论文详细信息
FEBS Letters
Orientation and interactions of dipolar molecules during transport through OmpF porin
Tieleman, D.Peter1  Robertson, Kindal M1 
[1] Department of Biological Sciences, University of Calgary, 2500 University Dr. NW, Calgary, AB, Canada T2N 1N4
关键词: Non-equilibrium molecular dynamics;    Sugar transport;    Single molecule experiment;    Alpha-methylglucose;    Membrane protein;    Escherichia coli;   
DOI  :  10.1016/S0014-5793(02)03173-3
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

The outer membrane of Gram-negative bacteria contains porins, large sieve-like proteins allowing small molecules to diffuse in and out of the periplasm. We have simulated transport of the dipolar molecules alanine and methylglucose through the OmpF porin from Escherichia coli using non-equilibrium steered molecular dynamics simulations in a realistic bilayer environment. Structural perturbation of the protein is minimal. During the permeation process, both alanine and methylglucose align strongly in the electric field in the eyelet region, where the adhesion force on the permeating molecule has a maximum. Binding of the permeating dipolar molecules in the eyelet region is not observed.

【 授权许可】

Unknown   

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