Evidence for the phosphorylation of enzyme IIglucose of the phosphoenolpyruvate—sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium

FEBS Letters

Waygood, E.Bruce¹  Peri, Krishna G.¹  Hans L, Kornberg² 

[1] Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada;Department of Biochemistry, University of Cambridge, Cambridge, England

关键词: Phosphoenolpyruvate—sugar phosphotransferase system;    Enzyme IIglucose;    Phosphoprotein;    Sugar transport;    Factor IIIglucose;    Membrane phosphorylation;   

DOI  :  10.1016/0014-5793(84)81367-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A membrane-bound phosphoprotein, of subunit Mr 48 000 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, has been found in Escherichia coli and Salmonella typhimurium. The phosphorylation of this protein is dependent upon phosphoenolpyruvate and the protein of the sugar phosphotransferase (PT) system: enzyme I, HPr, and IIIglucose. The membrane-bound phosphoprotein is identified as enzyme II^glucose. Other membrane-bound phosphoproteins, that are also dependent upon the PT system, remain to be identified.

【 授权许可】

Unknown