期刊论文详细信息
FEBS Letters | |
The regions of securin and cyclin B proteins recognized by the ubiquitination machinery are natively unfolded | |
Pascal, Steven M1  Hwang, William C1  Dutta, Kaushik1  Basavappa, Ravi1  Cox, Cathleen J1  Petri, Edward T1  Lin, Yaqiong1  | |
[1] Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Ave., Rochester, NY 14618, USA | |
关键词: Natively unfolded; Mitosis; Circular dichroism; Nuclear magnetic resonance; Cyclin B; Securin; APC/C; anaphase promoting complex/cyclosome; CD; circular dichroism; NMR; nuclear magnetic resonance; NOE; nuclear Overhauser effect; | |
DOI : 10.1016/S0014-5793(02)03246-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The proteins securin and cyclin B are destroyed in mitosis by the ubiquitin/proteasome system. This destruction is important to mitotic progression. The N-terminal regions of these proteins contain the sequence features recognized by the ubiquitination system. We have demonstrated using circular dichroism and 1-D and 2-D nuclear magnetic resonance that these rather substantial regions are natively unfolded. Based on these findings, we propose a model that helps to explain previously enigmatic observations.
【 授权许可】
Unknown
【 预 览 】
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RO201912020312157ZK.pdf | 214KB | download |