Human immune cells express ppMCH mRNA and functional MCHR1 receptor

FEBS Letters

Lakaye, Bernard²  Chanas, Grazyna²  Adamantidis, Antoine²  Zorzi, Willy¹  Grisar, Thierry²  Verlaet, Myriam²  Coumans, Bernard²  Heinen, Ernst¹ 

[1] Center for Human Histology, University of Liège, B-4020 Liège, Belgium;Center for Cellular and Molecular Neurobiology, University of Liège, 17 place Delcour, B-4020 Liège, Belgium

关键词: Melanin concentrating hormone;    Melanin concentrating hormone receptor;    Immune cells;    Proliferation;    cAMP;    MCH;    melanin-concentrating hormone;    ppMCH;    prepro-MCH;    MCHR;    MCH receptor;    m;    mouse;    h;    human;    PBMC;    peripheral blood mononuclear cell;   

DOI  :  10.1016/S0014-5793(02)03232-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Melanin-concentrating hormone (MCH) is highly expressed in the brain and modulates feeding behavior. It is also expressed in some peripheral tissues where its role remains unknown. We have investigated MCH function in human and mouse immune cells. RT-PCR analysis revealed a low expression of prepro-MCH and MCH receptor 1 (MCHR1) but not of MCHR2 transcript in tissular and peripheral blood immune cells. FACS and in vitro assay studies demonstrated that MCHR1 receptor expression on most cell types can trigger, in the presence of MCH, cAMP synthesis and calcium mobilization in peripheral blood mononuclear cells (PBMCs). Moreover, MCH treatment decreases the CD3-stimulated PBMC proliferation in vitro. Accordingly, our data indicate for the first time that MCH and MCHR1 may exert immunomodulatory functions.

【 授权许可】

Unknown