Characterization of the spermidine synthase‐related gene family in Arabidopsis thaliana

FEBS Letters

Komeda, Yoshibumi¹  Michael, Anthony J²  Imai, Akihiro¹  Hanzawa, Yoshie¹  Takahashi, Taku¹ 

[1] Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan;Division of Food Safety Science, Institute of Food Research, Colney Lane, Norwich NR4 7UH, UK

关键词: Gene expression;    Polyamine;    Spermidine synthase;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(02)03217-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Arabidopsis genome contains four genes that encode proteins similar to both spermidine synthase and spermine synthase of other organisms. Our previous study revealed that one of these genes, designated ACAULIS5 (ACL5), encodes spermine synthase and that its null mutation results in a severe defect in the elongation of stem internodes. Here we report the characterization of the other three genes, designated SPDS1, SPDS2 and SPDS3. Our results showed that SPDS1 and SPDS2 possess spermidine synthase activity in yeast spermidine synthase-deficient mutants, but the enzyme activity of SPDS3 remained to be determined. RNA gel blot analysis revealed that all of these genes are expressed in all plant organs but show different responses to exogenous plant hormones, suggesting that they are involved in different aspects of growth by modulating the contents of polyamines in plant cells.

【 授权许可】

Unknown