Spermine is not essential for survival of Arabidopsis

FEBS Letters

Sato, Shusei¹  Akiyama, Takashi³  Imai, Akihiro²  Takahashi, Taku²  Yamamoto, Kotaro T²  Tabata, Satoshi¹  Kato, Tomohiko¹ 

[1] Kazusa DNA Research Institute, Kisarazu, Chiba 292-0812, Japan;Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan;Department of Low Temperature Science, Hokkaido National Agricultural Experiment Station, Sapporo 062-8555, Japan

关键词: ACL5;    Polyamine;    Spermine synthase;    SPMS;    Arabidopsis thaliana;   

DOI  :  10.1016/S0014-5793(03)01395-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spermine is the final product of the polyamine biosynthetic pathway and is ubiquitously present in most organisms. The genome of Arabidopsis thaliana has two genes encoding spermine synthase: ACAULIS5 (ACL5), whose loss-of-function mutants show a severe defect in stem elongation, and SPMS. In order to elucidate the function of spermine in plants, we isolated a T-DNA insertion mutant of the SPMS gene. Free and conjugated spermine levels in the mutant, designated spms-1, were significantly decreased compared with those in the wild-type, but no obvious morphological phenotype was observed in spms-1 plants. We further confirmed that acl5-1 spms-1 double mutants contained no spermine. Surprisingly, acl5-1 spms-1 was fully as viable as the wild-type and showed no phenotype except for the reduced stem growth due to acl5-1. These results indicate that spermine is not essential for survival of Arabidopsis, at least under normal growth conditions.

【 授权许可】

Unknown