Novel strategy for anti‐HIV‐1 action: selective cytotoxic effect of N‐myristoyltransferase inhibitor on HIV‐1‐infected cells

FEBS Letters

Hamada, Hirotoshi¹  Misumi, Shogo¹  Takamune, Nobutoki¹  Shoji, Shozo¹ 

[1] Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-Honmachi, Kumamoto 862-0973, Japan

关键词: N-myristoyltransferase;    Myristoylation;    Human immunodeficiency virus type-1;    NMT;    N-myristoyltransferase;    ELISA;    enzyme-linked immunosorbent assay;    HIV-1;    human immunodeficiency virus type-1;    RT;    reverse transcriptase;    HAART;    highly active anti-retroviral therapy;   

DOI  :  10.1016/S0014-5793(02)03199-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-myristoyltransferase (NMT) is essential for the survival of eukaryotes and the production of infectious human immunodeficiency virus type-1(HIV-1) by the host cell. In this study, we found decreases in the mRNA levels of human NMT isoforms and the NMT activities in the course of HIV-1 infection in the human T-cell line, CEM. Investigating the cytotoxic effect of the novel synthetic NMT inhibitors on the chronic HIV-1 infected T-cell line, CEM/LAV-1, and the uninfected CEM, revealed that the cytotoxic effect was significantly selective for CEM/LAV-1. This was thought to be due to the difference between the NMT levels of the cell lines. In this paper, we propose that NMT may be a candidate target for anti-HIV-1-infected-cell agents.

【 授权许可】

Unknown