Estradiol receptor potentiates, in vitro, the activity of 5‐methylcytosine DNA glycosylase

FEBS Letters

Siegmann, Michel¹  Thiry, Stéphane¹  Jost, Jean-Pierre¹ 

[1] Friedrich Miescher Institute, Maulbeerstrasse 66, CH-4058 Basel, Switzerland

关键词: DNA demethylation;    Targeting demethylation;    Protein–protein interaction;    Hemi-methylated DNA;    Estradiol response element;   

DOI  :  10.1016/S0014-5793(02)03166-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

At a concentration of 5×10⁻⁹ M of hemi-methylated DNA (one order of magnitude below the K _m), MCF-7 (a human breast carcinoma cell line) nuclear extracts potentiate the activity of 5-methylcytosine DNA glycosylase (5-MCDG, alias G/T mismatch DNA glycosylase). Depending on the ratio between MCF-7 nuclear extracts and 5-MCDG, there is an up to 10-fold increase in 5-MCDG activity. The potentiation of 5-MCDG by MCF-7 nuclear extracts requires an estradiol response element adjacent to the hemi-methylated site. Depletion of the estradiol receptor from MCF-7 nuclear extracts with specific antibodies abolishes the potentiation of 5-MCDG activity. The estradiol receptor present in MCF-7 nuclear extracts can be precipitated with antibodies directed against 5-MCDG. Reciprocally, antibodies directed against the estradiol receptor precipitate 5-MCDG. The results indicate the formation of a complex between the estradiol receptor and 5-MCDG.

【 授权许可】

Unknown