Potentiation of hematopoietic cell migration with an IGF–interleukin‐3 fusion protein

FEBS Letters

DiFalco, Marcos R¹  Congote, Luis Fernando¹ 

[1] Endocrine Laboratory, McGill University Health Centre, 687 avenue des pins, ouest, Montreal, QC, Canada H3A 1A1

关键词: Mitogen-activated protein kinase;    Phosphatidylinositol-3 kinase;    Protein kinase C;    Rho kinase;    Fibronectin;    BOMIGF;    NQPQMVHTY-hIGF-II(9–67);    IL-3;    interleukin-3;    MAPK;    mitogen-activated protein kinase;    PI-3;    phosphatidylinositol-3;    PKC;    protein kinase C;   

DOI  :  10.1016/S0014-5793(02)03025-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A chimera of an N-terminally modified insulin growth factor (IGF)-II, NQPQMVHTY-hIGF-II(9–67) (BOMIGF), fused to interleukin-3 (IL-3) significantly improved the migration of CD34⁺ human hematopoietic cells with respect to the effects observed during co-stimulation with BOMIGF and IL-3. A phosphatidylinositol-3 (PI-3) kinase inhibitor specifically inhibited migration in the presence of the chimera, while no significant difference in the inhibition of migration was observed in the presence of a Rho kinase inhibitor. These results suggest a key role of the PI-3 kinase pathway in the potentiation of migration caused by the linkage of BOMIGF and IL-3.

【 授权许可】

Unknown