The C‐terminus of myogenin, but not MyoD, targets upregulation of MEF2C expression

FEBS Letters

Rogerson, Parker J¹  Jamali, Mina¹  Skerjanc, Ilona S¹ 

[1] Department of Biochemistry, Medical Sciences Building, The University of Western Ontario, London, ON, Canada N6A 5C1

关键词: Myogenesis;    P19 cell;    Embryonal carcinoma;    MyoD;    Myogenin;    Myocyte-specific enhancer binding factor 2C;    MyHC;    myosin heavy chain;    PGK;    phosphoglycerate kinase;    DMSO;    dimethylsulfoxide;   

DOI  :  10.1016/S0014-5793(02)03024-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The myogenic regulatory family of basic helix-loop-helix transcription factors, including MyoD and myogenin, functions cooperatively with the myocyte-specific enhancer binding factor 2 (MEF2) family during skeletal myogenesis. Previously, using aggregated P19 cells, we have shown that myogenin upregulates MEF2C expression while MyoD does not [Ridgeway et al., J. Biol. Chem. 275 (2000) 41–46]. In order to identify the domain of myogenin responsible for activating MEF2C expression, a series of chimeras of MyoD and myogenin were generated. Only chimeras containing the C-terminal region of myogenin were able to activate MEF2C in aggregated P19 cells, suggesting that the C-terminus of myogenin is responsible for the regulation of specific target genes.

【 授权许可】

Unknown