FEBS Letters | |
Identification of functional p53‐binding motifs in the mouse wig‐1 promoter | |
Wiman, Klas G1  Méndez-Vidal, Cristina1  Wilhelm, Margareta T1  | |
[1] Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Karolinska Institute, SE-171 76 Stockholm, Sweden | |
关键词: wig-1; p53-Binding site; Promoter; BM; binding motif; EMSA; electrophoresis mobility shift assay; MEF; mouse embryonic fibroblast; mt; mutant; ORF; open reading frame; TSS; transcription start site; wt; wild-type; | |
DOI : 10.1016/S0014-5793(02)03004-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We previously identified wig-1 as a p53-induced mouse gene that encodes a nuclear zinc finger protein with unknown function. To investigate whether wig-1 is a direct target of p53-dependent transactivation, a DNA fragment corresponding to the promoter region was cloned and sequenced. Three regions containing consensus p53-binding sites were identified. Two p53-binding motifs formed DNA–protein complexes with p53 and were able to drive p53-dependent transcription in a luciferase reporter assay. Our results demonstrate that wig-1 is a direct target of p53-mediated transcriptional transactivation.
【 授权许可】
Unknown
【 预 览 】
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RO201912020312028ZK.pdf | 195KB | download |