Identification of functional p53‐binding motifs in the mouse wig‐1 promoter

FEBS Letters

Wiman, Klas G¹  Méndez-Vidal, Cristina¹  Wilhelm, Margareta T¹ 

[1] Department of Oncology-Pathology, Cancer Center Karolinska (CCK), Karolinska Institute, SE-171 76 Stockholm, Sweden

关键词: wig-1;    p53-Binding site;    Promoter;    BM;    binding motif;    EMSA;    electrophoresis mobility shift assay;    MEF;    mouse embryonic fibroblast;    mt;    mutant;    ORF;    open reading frame;    TSS;    transcription start site;    wt;    wild-type;   

DOI  :  10.1016/S0014-5793(02)03004-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously identified wig-1 as a p53-induced mouse gene that encodes a nuclear zinc finger protein with unknown function. To investigate whether wig-1 is a direct target of p53-dependent transactivation, a DNA fragment corresponding to the promoter region was cloned and sequenced. Three regions containing consensus p53-binding sites were identified. Two p53-binding motifs formed DNA–protein complexes with p53 and were able to drive p53-dependent transcription in a luciferase reporter assay. Our results demonstrate that wig-1 is a direct target of p53-mediated transcriptional transactivation.

【 授权许可】

Unknown