Identification of atypical (non‐AT1, non‐AT2) angiotensin binding sites with high affinity for angiotensin I on IEC‐18 rat intestinal epithelial cells

FEBS Letters

Smith, Roger D.¹ 

[1] Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge, CB2 2QQ, UK

关键词: Angiotensin I;    Atypical angiotensin recepter;    Angiotensin receptor antagonist;    IEC-18 cell;    Intestinal epithelium;    Ang;    angiotensin;    AT;    angiotensin receptor;    BM;    binding medium;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    foetal calf serum;    HEPES;    N-2-hydroxyethyl-piperazine-N'-2-ethane-ulphonic acid;   

DOI  :  10.1016/0014-5793(95)01039-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Specific high-affinity (Kd = 3.4 nM) binding sites for ¹²⁵I-labelled angiotensin I ([¹²⁵I]Ang I) were identified on an epithelial cell line (IEC-18) derived from the rat small intestine. The sites, which also have high affinity for Ang II, are insensitive to both AT₁- and AT₂-specific angiotensin receptor antagonists, The rank order of potency with which various angiotensin peptides inhibited[¹²⁵I]Ang I binding to the cells (Ang I ≥ Ang II > Ang(1–7) > [Sar¹,Ile⁸]-Ang II > Ang(3–8) > Ang III) also distinguishes these sites from AT₁ and AT₂ angiotensin receptors.

【 授权许可】

Unknown