Zinc accelerates dengue virus type 2‐induced apoptosis in Vero cells

FEBS Letters

AbuBakar, Sazaly¹  Shafee, Norazizah¹ 

[1] Department of Medical Microbiology, Faculty of Medicine, University Malaya, Kuala Lumpur 50603, Malaysia

关键词: Apoptosis;    Cation;    Dengue;    Vero;    Zinc;    DENV-2;    dengue virus type 2;    m.o.i.;    multiplicity of infection;    TUNEL;    terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling;   

DOI  :  10.1016/S0014-5793(02)02991-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dengue virus type 2 (DENV-2) infection induced apoptotic cellular DNA fragmentation in Vero cells within 8 days of infection. The addition of high concentrations of extracellular Zn²⁺ but not Ca²⁺, Mg²⁺ or Mn²⁺ to the cell culture medium hastened the detection of apoptosis to within 4 h after infection. No apoptotic cellular DNA fragmentation was detected in the cell culture treated with Zn²⁺ alone or infected with heat- or ultraviolet light-inactivated DENV-2 in the presence of Zn²⁺. These results suggest that (i) apoptosis is induced in African green monkey kidney cells infected with live DENV-2 and (ii) the addition of high extracellular Zn²⁺ accelerates detection of apoptosis in the DENV-2-infected cells.

【 授权许可】

Unknown