Serine 774 and amino acids 296 to 437 comprise the major C4 determinants of the C4 phosphoenolpyruvate carboxylase of Flaveria trinervia

FEBS Letters

Engelmann, Sascha²  Bläsing, Oliver E²  Svensson, Per¹  Westhoff, Peter² 

[1] Department of Plant Biology, The Swedish University of Agricultural Sciences, P.O. Box 7080, SE-750 07 Uppsala, Sweden;Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany

关键词: Phosphoenolpyruvate carboxylase;    Evolution;    C4 photosynthesis;    Flaveria;    PEPCase;    phosphoenolpyruvate carboxylase;    Glc6P;    glucose 6-phosphate;    ppc;    phosphoenolpyruvate carboxylase gene;   

DOI  :  10.1016/S0014-5793(02)02975-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

C4 phosphoenolpyruvate carboxylases have evolved several times independently from non-photosynthetic C3 ancestral enzymes. To identify C4-specific determinants at the amino acid level the two orthologous ppcA PEPCases of Flaveria trinervia (C4) and Flaveria pringlei (C3) were used as a model system. In a previous publication [Bläsing et al., J. Biol. Chem. 275 (2000) 27917–27923] it was reported that the serine at position 774 is an invariant residue in all C4 phosphoenolpyruvate carboxylases. Here we show by swapping experiments and site-directed mutagenesis that the serine 774 and amino acids 296–437 explain two thirds of the C4 characteristic phosphoenolpyruvate saturation kinetics when investigated in the C3 background. In addition, the results indicate that the determinants functionally interact with each other.

【 授权许可】

Unknown