Further analysis of cDNA clones for maize phosphoenolpyruvate carboxylase involved in C4 photosynthesis Nucleotide sequence of entire open reading frame and evidence for polyadenylation of mRNA at multiple sites in vivo

FEBS Letters

Izui, Katsura¹  Yamaguchi, Yasunori¹  Yanagisawa, Shuichi¹  Katsuki, Hirohiko¹  Shigesada, Katsuya² 

[1] Department of Chemistry, Faculty of Science, Kyoto University, Kyoto 606, Japan;Department of Biochemistry, Institute for Virus Research, Kyoto University, Kyoto 606, Japan

关键词: Phosphoenolpyruvate carboxylase;    cDNA cloning;    C4 photosynthesis;    Polyadenylation;    (Maize);    PEPC;    phosphoenolpyruvate carboxylase;    Kb;    kilo base pair;    bp;    base pair;    nt;    nucleotide;   

DOI  :  10.1016/0014-5793(88)80807-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four clones of cDNA for phosphoenolpyruvate carboxylase [EC 4.1.1.31] were obtained from a maize green leaf cDNA library by colony hybridization. The largest cDNA was of full-length (3335 nucleotides), being 243 nucleotides longer than the cDNA cloned previously [(1986) Nucleic Acids Res. 14, 1615–1628]. Alignment of the sequence for the N-terminal coding region found in two of the four clones with the sequence reported previously, established the sequence of the entire coding region for the enzyme. The sequencing of 3′-untranslated region of the clones revealed that the poly(A) tract is attached at multiple sites in vivo.

【 授权许可】

Unknown