Cytoskeleton‐related trafficking of the EAAC1 glutamate transporter after activation of the Gq/11‐coupled neurotensin receptor NTS1

FEBS Letters

Maloteaux, Jean-Marie¹  Hermans, Emmanuel¹  Najimi, Mustapha¹ 

[1]Laboratoire de Pharmacologie Expérimentale, Université Catholique de Louvain, Avenue Hippocrate 54, 1200 Brussels, Belgium

关键词: Trafficking;    Glioma;    G protein-coupled receptor;    Phospholipase C;    EAAC1;    EAAC1;    neuronal glutamate transporter;    NT;    neurotensin;    NTS1;    high-affinity neurotensin receptor;    PKC;    protein kinase C;    PLC;    phospholipase C;    PMA;    phorbol 12-myristate 13-acetate;    PI3-K;    phosphatidylinositol 3-kinase;    C6;    rat glioma cells;    C6-NTS1;    stably transfected C6 cells expressing the rat NTS1 receptor;    PAO;    phenylarsine oxide;   

DOI  :  10.1016/S0014-5793(02)02981-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possible modulation of the glutamate transporter EAAC1 by a class A G protein-coupled receptor was studied in transfected C6 glioma cells stably expressing the high-affinity neurotensin receptor NTS1. Brief exposure (5 min) to neurotensin increased Na⁺-dependent D-[³H]aspartate uptake by about 70%. The effect of neurotensin was found to result from an increase in cell surface expression of EAAC1 and accordingly, cytochalasin D and colchicine were shown to block the effect of neurotensin on aspartate uptake, suggesting that the cytoskeleton participates in this regulation. Neither protein kinase C nor phosphatidylinositol 3-kinase activities, two intracellular signaling pathways known to modulate EAAC1, was required for EAAC1-mediated aspartate transport regulation by neurotensin. Together, these results provide evidence for an acute regulation of EAAC1 trafficking after activation of a G protein-coupled receptor.

【 授权许可】

Unknown