Magic‐angle spinning 31P NMR spectroscopy of condensed phosphates in parasitic protozoa: visualizing the invisible

FEBS Letters

Moreno, Silvia N.J¹  Urbina, Julio A²  Moreno, Benjamin²  Bailey, Brian N¹  Docampo, Roberto¹  Rodrigues, Claudia O¹  Oldfield, Eric² 

[1] Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana-Champaign, 2100 South Lincoln Avenue, Urbana, IL 61802, USA;Departments of Chemistry and Biophysics, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL 61801, USA

关键词: Trypanosome;    Acidocalcisome;    Polyphosphate;    Solid state;    Nuclear magnetic resonance;   

DOI  :  10.1016/S0014-5793(02)02977-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the results of a solid-state ³¹P nuclear magnetic resonance (NMR) spectroscopic investigation of the acidocalcisome organelles from Trypanosoma brucei (bloodstream form), Trypanosoma cruzi and Leishmania major (insect forms). The spectra are characterized by a broad envelope of spinning sidebands having isotropic chemical shifts at ∼0, −7 and −21 ppm. These resonances are assigned to orthophosphate, terminal (α) phosphates of polyphosphates and bridging (β) phosphates of polyphosphates, respectively. The average polyphosphate chain length is ∼3.3 phosphates. Similar results were obtained with whole L. major promastigotes. ³¹P NMR spectra of living L. major promastigotes recorded under conventional solution NMR conditions had spectral intensities reduced with respect to solution-state NMR spectra of acid extracts, consistent with the invisibility of the solid-state phosphates. These results show that all three parasites contain large stores of condensed phosphates which can be visualized by using magic-angle spinning NMR techniques.

【 授权许可】

Unknown