Presence of a Na+/H+ exchanger in acidocalcisomes of Leishmania donovani and their alkalization by anti‐leishmanial drugs

FEBS Letters

Catisti, Rosana¹  Rodrigues, Claudia O.¹  Vercesi, Anibal E.¹  Docampo, Roberto¹ 

[1] Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 S. Lincoln Avenue, Urbana, IL 61802, USA

关键词: Acidocalcisome;    Na+/H+ exchanger;    3;    5-Dibutyl-4-hydroxytoluene;    Volutin;    Leishmania donovani;   

DOI  :  10.1016/S0014-5793(00)01531-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acidocalcisomes are acidic vacuoles present in trypanosomatids that contain most of the cellular calcium. The data presented here demonstrate that Leishmania donovani acidocalcisomes possess a Na⁺/H⁺ exchanger. 3,5-Dibutyl-4-hydroxytoluene, in the concentration range of 0–20 μM, inhibited the Na⁺/H⁺ exchanger, and strongly stimulated the activity of the vacuolar H⁺-ATPase responsible for vacuolar acidification. As occurs with Na⁺, the cationic anti-leishmanial drugs pentamidine, WR-6026, and chloroquine promoted a fast and extensive alkalization of the L. donovani acidocalcisomes.

【 授权许可】

Unknown