Mitochondria hyperpolarization is an early event in oxidized low‐density lipoprotein‐induced apoptosis in Caco‐2 intestinal cells

FEBS Letters

Scazzocchio, Beatrice¹  Malorni, Walter³  Matarrese, Paola³  Giovannini, Claudio¹  Masella, Roberta¹  Sanchez, Massimo² 

[1] Department of Metabolism and Biochemical Pathology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy;Department of Cell Biology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy;Department of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy

关键词: Caco-2;    Mitochondrial membrane potential;    Apoptotic pathway;    oxLDL;    DEVD-CHO;    Asp-Glu-Val-Asp-aldehyde;    DiOC6;    3;    3′-dihexyloxacarbocyanine iodide;    DHR 123;    dihydrorhodamine 123;    ΔΨ;    mitochondrial membrane potential;    FCCP;    carbonyl-cyanide p-(trifluoro-methoxy) phenylhydrazone;    HE;    hydroetidine;    IETD-CHO;    Ile-Glu-Thr-Asp-aldehyde;    JC-1;    5;    5′;    6;    6′-tetrachloro-1;    1′;    3;    3′tetraethylbenzimidazolcarbocyanineiodide;    LDL;    low-density lipoproteins;    LEHD-CHO;    Leu-Glu-His-Asp-aldehyde;    oxLDL;    oxidized low-density lipoproteins;    ROS;    reactive oxygen species;    TMRM;    tetramethylrhodamine ester;   

DOI  :  10.1016/S0014-5793(02)02972-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the mechanisms underlying the pro-apoptotic activity exerted by oxidized low-density lipoproteins (oxLDL) in Caco-2 intestinal cells, a cell line which retains many morphological and enzymatic features typical of normal human enterocytes. We found that: (i) oxLDL induced mitochondrial-mediated apoptosis by provoking first an increase in mitochondrial membrane potential, followed, later, by the typical apoptosis-associated depolarization (type II apoptosis); accordingly, (ii) caspase-9 inhibition significantly hindered apoptosis while caspase-8 inhibition did not; and finally (iii) dietary phenolic antioxidizing compounds exerted a significant protective antiapoptotic activity. These results point to mitochondrial hyperpolarization as ‘sensitizing feature’ in apoptotic proneness of Caco-2 intestinal cells to oxLDL exposure.

【 授权许可】

Unknown